Sleep deprivation and divergent toll-like receptor-4 activation of cellular inflammation in aging.

TitleSleep deprivation and divergent toll-like receptor-4 activation of cellular inflammation in aging.
Publication TypeJournal Article
Year of Publication2015
AuthorsCarroll JE, Carrillo C, Olmstead R, Witarama T, Breen EC, Yokomizo M, Seeman T, Irwin MR
Date Published2015
KeywordsAdult, Aged, Aged, 80 and over, Aging, Communicable Diseases, Cytokines, Female, Humans, Inflammation, Interleukin-6, Male, Middle Aged, Monocytes, Sleep, Sleep Deprivation, Toll-Like Receptor 4, Tumor Necrosis Factor-alpha

OBJECTIVES: Sleep disturbance and aging are associated with increases in inflammation, as well as increased risk of infectious disease. However, there is limited understanding of the role of sleep loss on age-related differences in immune responses. This study examines the effects of sleep deprivation on toll-like receptor activation of monocytic inflammation in younger compared to older adults.

DESIGN, SETTING, AND PARTICIPANTS: Community-dwelling adults (n = 70) who were categorized as younger (25-39 y old, n = 21) and older (60-84 y old, n = 49) participants, underwent a sleep laboratory-based experimental partial sleep deprivation (PSD) protocol including adaptation, an uninterrupted night of sleep, sleep deprivation (sleep restricted to 03:00-07:00), and recovery.

MEASUREMENT AND RESULTS: Blood samples were obtained each morning to measure toll-like receptor-4 activation of monocyte intracellular production of the inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Partial sleep deprivation induced a significant increase in the production of IL-6 and/or TNF-α that persisted after a night of recovery sleep (F(2,121.2) = 3.8, P < 0.05). Age moderated the effects of sleep loss, such that younger adults had an increase in inflammatory cytokine production that was not present in older adults (F(2,121.2) = 4.0, P < 0.05).

CONCLUSION: Older adults exhibit reduced toll-like receptor 4 stimulated cellular inflammation that, unlike in younger adults, is not activated after a night of partial sleep loss. Whereas sleep loss increases cellular inflammation in younger adults and may contribute to inflammatory disorders, blunted toll-like receptor activation in older adults may increase the risk of infectious disease seen with aging.

Alternate JournalSleep
PubMed ID25325509
PubMed Central IDPMC4288601
Grant ListP30 AG028748 / AG / NIA NIH HHS / United States
P30-AG028748 / AG / NIA NIH HHS / United States
R01 CA160245-01 / CA / NCI NIH HHS / United States
R01 DA032922-01 / DA / NIDA NIH HHS / United States
R01 HL095799 / HL / NHLBI NIH HHS / United States
R01-AG026364 / AG / NIA NIH HHS / United States
R01-AG034588 / AG / NIA NIH HHS / United States
R01-CA119159 / CA / NCI NIH HHS / United States
T32-MH19925 / MH / NIMH NIH HHS / United States
UL1TR000124 / TR / NCATS NIH HHS / United States