Progress in concurrent analysis of loss of heterozygosity and comparative genomic hybridization utilizing high density single nucleotide polymorphism arrays.
Title | Progress in concurrent analysis of loss of heterozygosity and comparative genomic hybridization utilizing high density single nucleotide polymorphism arrays. |
Publication Type | Journal Article |
Year of Publication | 2005 |
Authors | Zhou X, Rao NP, Cole SW, Mok SC, Chen Z, Wong DT |
Journal | Cancer Genet Cytogenet |
Volume | 159 |
Issue | 1 |
Pagination | 53-7 |
Date Published | 2005 May |
ISSN | 0165-4608 |
Keywords | Genome, Human, Humans, Loss of Heterozygosity, Microsatellite Repeats, Neoplasms, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Polymorphism, Single Nucleotide |
Abstract | Genetic aberrations, such as deletions and amplifications are among the major pathogenetic mechanisms underlying many medical disorders. Analysis of chromosomal aberrations is particularly important in cancer research, where amplifications of oncogenes and deletions of tumor suppressor genes are major steps in the "multi-hit" process of tumorigenesis. Genome-wide molecular biological analyses, such as loss of heterozygosity (LOH) profiling and comparative genomic hybridization (CGH) have significantly enhanced our ability to detect chromosomal aberrations in cancer cells and assess their role in tumorigenesis. The recent introduction of high-density oligonucleotide arrays for measuring single nucleotide polymorphisms (SNP) has sparked a new wave of high-resolution genetic mapping studies, including LOH and CGH applications on various cancer types. This review highlights recent progress on concurrent LOH and CGH analyses utilizing high density SNP arrays and their application in cancer research. |
DOI | 10.1016/j.cancergencyto.2004.09.014 |
Alternate Journal | Cancer Genet. Cytogenet. |
PubMed ID | 15860358 |
Grant List | K22 DE014847 / DE / NIDCR NIH HHS / United States P50CA165009 / CA / NCI NIH HHS / United States R01 AI52737 / AI / NIAID NIH HHS / United States R01 DE015970-01 / DE / NIDCR NIH HHS / United States R21 AI49135 / AI / NIAID NIH HHS / United States R33CA103595 / CA / NCI NIH HHS / United States |