Opioid treatment of experimental pain activates nuclear factor-κB.

TitleOpioid treatment of experimental pain activates nuclear factor-κB.
Publication TypeJournal Article
Year of Publication2015
AuthorsCompton P, Griffis C, Breen ECrabb, Torrington M, Sadakane R, Tefera E, Irwin MR
JournalJ Opioid Manag
Volume11
Issue2
Pagination115-25
Date Published2015 Mar-Apr
ISSN1551-7489
KeywordsAdult, Analgesics, Opioid, Female, Fentanyl, Follow-Up Studies, Healthy Volunteers, Humans, Injections, Intravenous, Male, Monocytes, NF-kappa B, Pain, Signal Transduction
Abstract

OBJECTIVE: To determine the independent and combined effects of pain and opioids on the activation of an early marker of inflammation, nuclear factor-κB (NF-κB).

DESIGN: NF-κB activation was compared within-subjects following four randomly ordered experimental sessions of opioid-only (intravenous fentanyl 1 μg/kg), painonly (cold-pressor), opioid + pain, and a resting condition.

SETTING: University General Clinical Research Center.

PARTICIPANTS: Twenty-one (11 female) healthy controls.

INTERVENTIONS: Following exposure to treatment (fentanyl administration and/or cold-pressor pain), blood samples for NF-κB analysis were obtained.

MAIN OUTCOME MEASURES: Intracellular levels of activated NF-κB, in unstimulated and stimulated peripheral blood mononuclear cells at 15 and 30 minutes.

RESULTS: Neither pain nor opioid administration alone effected NF-κB levels in cell populations; however, the combination of treatments induced significant increases of NF-κB in stimulated peripheral blood mononuclear cell, lymphocytes, and monocytes.

CONCLUSIONS: The combination of acute pain with opioids, as occurs in clinical situations, activates a key transcription factor involved in proinflammatory responses.

DOI10.5055/jom.2015.0261
Alternate JournalJ Opioid Manag
PubMed ID25901477
PubMed Central IDPMC4507428
Grant ListP30 AG028748 / AG / NIA NIH HHS / United States
P30-AG028748 / AG / NIA NIH HHS / United States
R01 AG026364 / AG / NIA NIH HHS / United States
R01 AG034588 / AG / NIA NIH HHS / United States
R01 CA119159 / CA / NCI NIH HHS / United States
R01 CA160245 / CA / NCI NIH HHS / United States
R01 CA160245-01 / CA / NCI NIH HHS / United States
R01 DA032922 / DA / NIDA NIH HHS / United States
R01 DA032922-01 / DA / NIDA NIH HHS / United States
R01 HL095799 / HL / NHLBI NIH HHS / United States
R01-AG026364 / AG / NIA NIH HHS / United States
R01-AG034588 / AG / NIA NIH HHS / United States
R01-CA119159 / CA / NCI NIH HHS / United States
R01HL095799 / HL / NHLBI NIH HHS / United States
R21 DA027558 / DA / NIDA NIH HHS / United States
R21-DA027558-01 / DA / NIDA NIH HHS / United States
UL1 RR031975 / RR / NCRR NIH HHS / United States
UL1 TR000124 / TR / NCATS NIH HHS / United States
UL1TR000124 / TR / NCATS NIH HHS / United States