Opioid treatment of experimental pain activates nuclear factor-κB.
Title | Opioid treatment of experimental pain activates nuclear factor-κB. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Compton P, Griffis C, Breen ECrabb, Torrington M, Sadakane R, Tefera E, Irwin MR |
Journal | J Opioid Manag |
Volume | 11 |
Issue | 2 |
Pagination | 115-25 |
Date Published | 2015 Mar-Apr |
ISSN | 1551-7489 |
Keywords | Adult, Analgesics, Opioid, Female, Fentanyl, Follow-Up Studies, Healthy Volunteers, Humans, Injections, Intravenous, Male, Monocytes, NF-kappa B, Pain, Signal Transduction |
Abstract | OBJECTIVE: To determine the independent and combined effects of pain and opioids on the activation of an early marker of inflammation, nuclear factor-κB (NF-κB). DESIGN: NF-κB activation was compared within-subjects following four randomly ordered experimental sessions of opioid-only (intravenous fentanyl 1 μg/kg), painonly (cold-pressor), opioid + pain, and a resting condition. SETTING: University General Clinical Research Center. PARTICIPANTS: Twenty-one (11 female) healthy controls. INTERVENTIONS: Following exposure to treatment (fentanyl administration and/or cold-pressor pain), blood samples for NF-κB analysis were obtained. MAIN OUTCOME MEASURES: Intracellular levels of activated NF-κB, in unstimulated and stimulated peripheral blood mononuclear cells at 15 and 30 minutes. RESULTS: Neither pain nor opioid administration alone effected NF-κB levels in cell populations; however, the combination of treatments induced significant increases of NF-κB in stimulated peripheral blood mononuclear cell, lymphocytes, and monocytes. CONCLUSIONS: The combination of acute pain with opioids, as occurs in clinical situations, activates a key transcription factor involved in proinflammatory responses. |
DOI | 10.5055/jom.2015.0261 |
Alternate Journal | J Opioid Manag |
PubMed ID | 25901477 |
PubMed Central ID | PMC4507428 |
Grant List | P30 AG028748 / AG / NIA NIH HHS / United States P30-AG028748 / AG / NIA NIH HHS / United States R01 AG026364 / AG / NIA NIH HHS / United States R01 AG034588 / AG / NIA NIH HHS / United States R01 CA119159 / CA / NCI NIH HHS / United States R01 CA160245 / CA / NCI NIH HHS / United States R01 CA160245-01 / CA / NCI NIH HHS / United States R01 DA032922 / DA / NIDA NIH HHS / United States R01 DA032922-01 / DA / NIDA NIH HHS / United States R01 HL095799 / HL / NHLBI NIH HHS / United States R01-AG026364 / AG / NIA NIH HHS / United States R01-AG034588 / AG / NIA NIH HHS / United States R01-CA119159 / CA / NCI NIH HHS / United States R01HL095799 / HL / NHLBI NIH HHS / United States R21 DA027558 / DA / NIDA NIH HHS / United States R21-DA027558-01 / DA / NIDA NIH HHS / United States UL1 RR031975 / RR / NCRR NIH HHS / United States UL1 TR000124 / TR / NCATS NIH HHS / United States UL1TR000124 / TR / NCATS NIH HHS / United States |