Novel orphanin FQ/nociceptin transcripts are expressed in human immune cells.

TitleNovel orphanin FQ/nociceptin transcripts are expressed in human immune cells.
Publication TypeJournal Article
Year of Publication2002
AuthorsArjomand J, Cole S, Evans CJ
JournalJ Neuroimmunol
Date Published2002 Sep
KeywordsAlternative Splicing, Amino Acid Sequence, Antigens, CD19, B-Lymphocytes, Base Sequence, Cell Culture Techniques, Gene Expression, Gene Expression Regulation, Humans, Lymphocytes, Molecular Sequence Data, Opioid Peptides, Promoter Regions, Genetic, Receptors, Opioid, RNA Splice Sites, RNA, Messenger, Spleen, Transcriptional Activation

The opioid-like receptor (NOP) is widely expressed throughout the human immune system. Here, we report that human peripheral blood lymphocytes (PBLs) express transcripts encoding the NOP receptor agonist, orphanin FQ/nociceptin (OFQ/N). OFQ/N transcripts in resting PBLs were restricted to CD19+B cells and contained a novel 5' exon (ImEx2b), replacing exons 1 and 2 found in neuronal transcripts. Translation of ImEx2b-containing transcripts resulted in truncated OFQ/N precursors lacking a classical signal peptide. Mitogen activation of PBLs dramatically up-regulated neuronal-like transcripts, predominantly in CD3+T cells. Overall, this suggests different promoters direct specific OFQ/N transcript expression in immune cells.

Alternate JournalJ. Neuroimmunol.
PubMed ID12225892
Grant ListDA-05010 / DA / NIDA NIH HHS / United States
P50 DA005010 / DA / NIDA NIH HHS / United States
T32-MH17140 / MH / NIMH NIH HHS / United States