Leptin and cellular and innate immunity in abstinent alcoholics and controls.

TitleLeptin and cellular and innate immunity in abstinent alcoholics and controls.
Publication TypeJournal Article
Year of Publication2003
AuthorsMotivala SJ, Dang J, Obradovic T, Meadows GG, Butch AW, Irwin MR
JournalAlcohol Clin Exp Res
Date Published2003 Nov
KeywordsAdult, Alcoholism, Analysis of Variance, Humans, Immunity, Cellular, Leptin, Male, Middle Aged, Temperance

BACKGROUND: Basic studies indicate that in vitro and in vivo doses of leptin modulate cellular immune responses. Given evidence that concentrations of leptin are altered in alcoholics who also show immune abnormalities, this study examined the relationships between circulating levels of leptin and markers of cellular and innate immunity.

METHODS: Circulating levels of leptin, natural killer cell (NK) activity, interleukin-2 (IL-2)-stimulated NK activity, and concanavalin A-stimulated production of IL-2, IL-6, IL-10, and IL-12 were compared between abstinent DSM-IV alcohol-dependent men (n = 27) and age- and gender-matched controls (n = 34).

RESULTS: As compared with controls, alcoholics showed lower NK activity (p < 0.01) and a trend for lower levels of leptin (p = 0.055). In the total sample, leptin predicted NK activity (beta = 0.33; p < 0.05) after controlling for the confounding influence of body mass index, alcohol intake, and smoking. Leptin was not correlated with any of the cytokine measures. To examine whether the effects of leptin were mediated by its direct action on NK, additional studies examined in vitro effects of leptin on NK activity in healthy volunteers (n = 10); leptin doses (0.1, 1, and 10 nM) yielded levels of NK activity comparable to those with media alone.

CONCLUSIONS: These data show that circulating levels of leptin are associated with NK activity in humans and suggest that abnormal in vivo concentrations of leptin may contribute to the declines of NK activity in alcoholics who are at risk for infectious diseases.

Alternate JournalAlcohol. Clin. Exp. Res.
PubMed ID14634499
Grant ListAA07293 / AA / NIAAA NIH HHS / United States
AA10215 / AA / NIAAA NIH HHS / United States
AA13239 / AA / NIAAA NIH HHS / United States
AG18367 / AG / NIA NIH HHS / United States
AR/AG41867 / AR / NIAMS NIH HHS / United States
AT00255 / AT / NCCIH NIH HHS / United States
MH55253 / MH / NIMH NIH HHS / United States
T32 AA07557 / AA / NIAAA NIH HHS / United States
T32 MH17140 / MH / NIMH NIH HHS / United States