Human immunodeficiency virus type 1 infection and replication in normal human oral keratinocytes.
Title | Human immunodeficiency virus type 1 infection and replication in normal human oral keratinocytes. |
Publication Type | Journal Article |
Year of Publication | 2003 |
Authors | Liu X, Zha J, Chen H, Nishitani J, Camargo P, Cole SW, Zack JA |
Journal | J Virol |
Volume | 77 |
Issue | 6 |
Pagination | 3470-6 |
Date Published | 2003 Mar |
ISSN | 0022-538X |
Keywords | Cells, Cultured, DNA, Viral, HIV Infections, HIV-1, Humans, Keratinocytes, Leukocytes, Mononuclear, Mouth Mucosa, Polymerase Chain Reaction, Proviruses, Receptors, HIV, Virus Replication |
Abstract | Recent epidemiologic studies show increasing human immunodeficiency virus type 1 (HIV-1) transmission through oral-genital contact. This paper examines the possibility that normal human oral keratinocytes (NHOKs) might be directly infected by HIV or might convey infectious HIV virions to adjacent leukocytes. PCR analysis of proviral DNA constructs showed that NHOKs can be infected by CXCR4-tropic (NL4-3 and ELI) and dualtropic (89.6) strains of HIV-1 to generate a weak but productive infection. CCR5-tropic strain Ba-L sustained minimal viral replication. Antibody inhibition studies showed that infection by CXCR4-tropic viral strains is mediated by the galactosylceramide receptor and the CXCR4 chemokine coreceptor. Coculture studies showed that infectious HIV-1 virions can also be conveyed from NHOKs to activated peripheral blood lymphocytes, suggesting a potential role of oral epithelial cells in the transmission of HIV infection. |
Alternate Journal | J. Virol. |
PubMed ID | 12610122 |
PubMed Central ID | PMC149546 |
Grant List | 5U42RR14616 / RR / NCRR NIH HHS / United States AI36059 / AI / NIAID NIH HHS / United States AI49135 / AI / NIAID NIH HHS / United States AI52737 / AI / NIAID NIH HHS / United States DE00430 / DE / NIDCR NIH HHS / United States P20 RR11145 / RR / NCRR NIH HHS / United States T32-DE07296 / DE / NIDCR NIH HHS / United States |