Human immunodeficiency virus type 1 infection and replication in normal human oral keratinocytes.

TitleHuman immunodeficiency virus type 1 infection and replication in normal human oral keratinocytes.
Publication TypeJournal Article
Year of Publication2003
AuthorsLiu X, Zha J, Chen H, Nishitani J, Camargo P, Cole SW, Zack JA
JournalJ Virol
Volume77
Issue6
Pagination3470-6
Date Published2003 Mar
ISSN0022-538X
KeywordsCells, Cultured, DNA, Viral, HIV Infections, HIV-1, Humans, Keratinocytes, Leukocytes, Mononuclear, Mouth Mucosa, Polymerase Chain Reaction, Proviruses, Receptors, HIV, Virus Replication
Abstract

Recent epidemiologic studies show increasing human immunodeficiency virus type 1 (HIV-1) transmission through oral-genital contact. This paper examines the possibility that normal human oral keratinocytes (NHOKs) might be directly infected by HIV or might convey infectious HIV virions to adjacent leukocytes. PCR analysis of proviral DNA constructs showed that NHOKs can be infected by CXCR4-tropic (NL4-3 and ELI) and dualtropic (89.6) strains of HIV-1 to generate a weak but productive infection. CCR5-tropic strain Ba-L sustained minimal viral replication. Antibody inhibition studies showed that infection by CXCR4-tropic viral strains is mediated by the galactosylceramide receptor and the CXCR4 chemokine coreceptor. Coculture studies showed that infectious HIV-1 virions can also be conveyed from NHOKs to activated peripheral blood lymphocytes, suggesting a potential role of oral epithelial cells in the transmission of HIV infection.

Alternate JournalJ. Virol.
PubMed ID12610122
PubMed Central IDPMC149546
Grant List5U42RR14616 / RR / NCRR NIH HHS / United States
AI36059 / AI / NIAID NIH HHS / United States
AI49135 / AI / NIAID NIH HHS / United States
AI52737 / AI / NIAID NIH HHS / United States
DE00430 / DE / NIDCR NIH HHS / United States
P20 RR11145 / RR / NCRR NIH HHS / United States
T32-DE07296 / DE / NIDCR NIH HHS / United States