Professor Mashudu Davhana-Maselesele was born at Balanganani village at Ha-Davhana being the third born child of the late Vhamusanda Vho DMK Davhana and Mrs Nyambeni Rhodah Davhana (nee‘ Nengovhela). She trained as a nurse and midwife at the then Venda Nursing College and served as a Professional nurse at Donald Fraser hospital. She obtained BA(Cur) Nursing Education and Community Health Nursing Science; Honours BA(Cur) in Nursing Education and Masters in Nursing Education all at UNISA. She completed her Doctoral degree in Programme Development at the University of Johannesburg. She was awarded a scholarship through Fogarty Funding to pursue her Postdoctoral studies at the University of California (Los Angeles) USA in collaboration with University of Limpopo. Her PostDoctoral studies focused on trauma research with special emphasis on Gender Based Violence. She also did Postgraduate Diploma in International Research Ethics in Southern Africa (IRENSA) at UCT.