Central CRH suppresses specific antibody responses: effects of beta-adrenoceptor antagonism and adrenalectomy.

TitleCentral CRH suppresses specific antibody responses: effects of beta-adrenoceptor antagonism and adrenalectomy.
Publication TypeJournal Article
Year of Publication2001
AuthorsFriedman EM, Irwin M
JournalBrain Behav Immun
Date Published2001 Mar
KeywordsAdjuvants, Immunologic, Adrenalectomy, Adrenergic beta-Antagonists, Animals, Corticotropin-Releasing Hormone, Hemocyanin, Immunoglobulin G, Immunoglobulin M, Male, Neuroimmunomodulation, Propranolol, Rats, Rats, Wistar, Receptors, Adrenergic, beta, Stress, Physiological, Sympathetic Nervous System

Central corticotropin releasing hormone (CRH)-induced activation of the sympathetic nervous system and/or the pituitary-adrenal axis is hypothesized to mediate suppression of in vivo specific antibody responses. To test whether beta-adrenergic receptor activation is involved in the immunosuppressive effects of central CRH, rats were pretreated with propranolol or saline before intracerebroventricular infusion of CRH and immunization with keyhole limpet hemocyanin (KLH). KLH (3 microg/kg) immunization induced significant increases in circulating levels of antigen-specific IgM and IgG. Central infusion of CRH (200 pmol) suppressed both IgM and IgG responses. Pretreatment with propranolol (20 mg/kg IP) reversed CRH-induced suppression of IgG responses but had no effect on IgM levels. To test whether adrenal activation also plays a role in the effects of KLH on specific antibody responses, a separate group of animals underwent adrenalectomy prior to CRH infusion and immunization with KLH. As compared to nonadrenalectomized control rats, adrenalectomized rats showed a reduction of antibody responses, and CRH failed to induce a further suppression of IgM or IgG responses in adrenalectomized animals. Collectively, these data suggest that beta-adrenoceptors mediate the suppression of primary antibody responses induced by central CRH. Moreover, the adrenals may promote optimal primary antibody responses after exposure to physiological levels of antigen.

Alternate JournalBrain Behav. Immun.
PubMed ID11259081
Grant List10881-01 / / PHS HHS / United States
46867 / / PHS HHS / United States