Selective detection of extracellular glutamate in brain tissue using microelectrode arrays coated with over-oxidized polypyrrole.

TitleSelective detection of extracellular glutamate in brain tissue using microelectrode arrays coated with over-oxidized polypyrrole.
Publication TypeJournal Article
Year of Publication2007
AuthorsWalker, E, Wang J, Hamdi N, Monbouquette HG, Maidment NT
JournalThe Analyst
Volume132
Issue11
Pagination1107-11
Date Published2007 Nov
ISSN0003-2654
KeywordsAnimals, Brain Chemistry, Electrochemistry, Extracellular Space, Glutamates, Humans, Microelectrodes, Polymers, Pyrroles
Abstract

We describe the selective measurement of extracellular glutamate concentration in rodent brain using ceramic-based platinum microelectrode arrays (MEAs) coated with electropolymerized, over-oxidized polypyrrole (OPP) as a permselective barrier to interference from dopamine and ascorbate. OPP-coated MEAs displayed similar sensitivity (97 +/- 9 nA microM(-1) cm(-2)) and response time (ca. 1 s) to glutamate as previously described Nafion-coated MEAs but, unlike Nafion-coated MEAs, were unresponsive to 5 microM dopamine. Such OPP-coated MEAs afforded selective detection of evoked glutamate release in the dopamine-rich striatum of the mouse brain.

DOI10.1111/j.1553-2712.2011.01024.x
Alternate JournalAnalyst