Descending projections from the dorsolateral pontine tegmentum to the paramedian reticular nucleus of the caudal medulla in the cat.

TitleDescending projections from the dorsolateral pontine tegmentum to the paramedian reticular nucleus of the caudal medulla in the cat.
Publication TypeJournal Article
Year of Publication1990
AuthorsShiromani, PJ, Lai YY, Siegel JM
JournalBrain research
Volume517
Issue1-2
Pagination224-8
Date Published1990 May 28
ISSN0006-8993
KeywordsAnimals, Cats, Choline O-Acetyltransferase, Cholinergic Fibers, Horseradish Peroxidase, Medulla Oblongata, Neural Pathways, pons, Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate, Wheat Germ Agglutinins
Abstract

We examined whether the dorsolateral pontine cholinergic cells project to the paramedian reticular nucleus (PRN) of the caudal medulla. In 3 cats, wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) was injected into the PRN and we noted cells in the dorsolateral pons that contained the HRP reaction product, cells that were immunolabeled for choline acetyltransferase (ChAT), and cells that contained the HRP reaction product and were ChAT positive. We found cholinergic projections from the pedunculopontine tegmental and laterodorsal tegmental nuclei to the PRN. This finding is consistent with studies indicating a cholinoceptive region in the medial medulla mediating suppression of muscle tone. Our results demonstrate that this medullary region has monosynaptic input from pontine neurons implicated in generating the atonia of rapid eye movement sleep.

Alternate JournalBrain Res.