Sleep deprivation decreases superoxide dismutase activity in rat hippocampus and brainstem.

TitleSleep deprivation decreases superoxide dismutase activity in rat hippocampus and brainstem.
Publication TypeJournal Article
Year of Publication2002
AuthorsRamanathan, L, Gulyani S, Nienhuis R, Siegel JM
JournalNeuroreport
Volume13
Issue11
Pagination1387-90
Date Published2002 Aug 7
ISSN0959-4965
KeywordsAnimals, Brain Stem, Glutathione Peroxidase, Hippocampus, Male, Oxidative Stress, Rats, Rats, Sprague-Dawley, sleep deprivation, Superoxide Dismutase
Abstract

Sleep deprivation by the disk-over-water technique results in a predictable syndrome of physiological changes in rats. It has been proposed that reactive oxygen species (ROS) may be responsible for some of these effects. A variety of antioxidative enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx) help to regulate the level of ROS. In this study we investigated the effects of prolonged (5-11 days) sleep deprivation on the activities of SOD and GPx as well as the metabolic activity of the mitochondria (using alamar blue) in several brain regions (cortex, hippocampus, hypothalamus, brainstem and cerebellum). We show that prolonged sleep deprivation significantly decreased Cu/Zn-SOD activity in the hippocampus and brainstem, suggesting an alteration in the metabolism of ROS resulting in oxidative stress.

DOI10.1111/j.1365-2869.2012.01023.x
Alternate JournalNeuroreport