Novel in vitro perfusion system for the determination of hypothalamic-pituitary-adrenal axis responses.
|Title||Novel in vitro perfusion system for the determination of hypothalamic-pituitary-adrenal axis responses.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Moidel, MA, Belz EE, Czambel KR, Rubin RT, Rhodes ME|
|Journal||Journal of pharmacological and toxicological methods|
|Date Published||2006 May-Jun|
|Keywords||Adrenal Glands, Adrenocorticotropic Hormone, Animals, Corticosterone, Corticotropin-Releasing Hormone, Female, Hydrogen-Ion Concentration, Hypothalamo-Hypophyseal System, hypothalamus, Male, Nicotine, Organ Culture Techniques, Perfusion, Pituitary Gland, Pituitary-Adrenal System, Rats, Rats, Sprague-Dawley, Reference Values, Sex Characteristics, Stimulation, Chemical, Temperature|
The hypothalamic-pituitary-adrenal (HPA) axis is a three-gland component of the endocrine system and a key modulator of the stress response. We have developed a novel in vitro perfusion system to enable the study of pharmacological and hormonal challenges to tissue components of the HPA axis. In vivo studies have shown functional sex differences (sexual diergism) in HPA responses to cholinergic drugs, and in the present in vitro study, we examine these differences at several levels of the HPA axis.
|Alternate Journal||J Pharmacol Toxicol Methods|