Novel in vitro perfusion system for the determination of hypothalamic-pituitary-adrenal axis responses.

TitleNovel in vitro perfusion system for the determination of hypothalamic-pituitary-adrenal axis responses.
Publication TypeJournal Article
Year of Publication2006
AuthorsMoidel, MA, Belz EE, Czambel KR, Rubin RT, Rhodes ME
JournalJournal of pharmacological and toxicological methods
Volume53
Issue3
Pagination264-71
Date Published2006 May-Jun
ISSN1056-8719
KeywordsAdrenal Glands, Adrenocorticotropic Hormone, Animals, Corticosterone, Corticotropin-Releasing Hormone, Female, Hydrogen-Ion Concentration, Hypothalamo-Hypophyseal System, hypothalamus, Male, Nicotine, Organ Culture Techniques, Perfusion, Pituitary Gland, Pituitary-Adrenal System, Rats, Rats, Sprague-Dawley, Reference Values, Sex Characteristics, Stimulation, Chemical, Temperature
Abstract

The hypothalamic-pituitary-adrenal (HPA) axis is a three-gland component of the endocrine system and a key modulator of the stress response. We have developed a novel in vitro perfusion system to enable the study of pharmacological and hormonal challenges to tissue components of the HPA axis. In vivo studies have shown functional sex differences (sexual diergism) in HPA responses to cholinergic drugs, and in the present in vitro study, we examine these differences at several levels of the HPA axis.

Alternate JournalJ Pharmacol Toxicol Methods