Muscle tone facilitation and inhibition after orexin-a (hypocretin-1) microinjections into the medial medulla.

TitleMuscle tone facilitation and inhibition after orexin-a (hypocretin-1) microinjections into the medial medulla.
Publication TypeJournal Article
Year of Publication2002
AuthorsMileykovskiy, BY, Kiyashchenko LI, Siegel JM
JournalJournal of neurophysiology
Volume87
Issue5
Pagination2480-9
Date Published2002 May
ISSN0022-3077
KeywordsAnesthetics, Local, Animals, Carrier Proteins, Electric Stimulation, Electromyography, Intracellular Signaling Peptides and Proteins, Lidocaine, Medulla Oblongata, Mesencephalon, Microinjections, Muscle Tonus, Muscle, Skeletal, Neuropeptides, pons, Rats, Rats, Wistar, Reticular Formation, Sympathomimetics
Abstract

Orexins/hypocretins are synthesized in neurons of the perifornical, dorsomedial, lateral, and posterior hypothalamus. A loss of hypocretin neurons has been found in human narcolepsy, which is characterized by sudden loss of muscle tone, called cataplexy, and sleepiness. The normal functional role of these neurons, however, is unclear. The medioventral medullary region, including gigantocellular reticular nucleus, alpha (GiA) and ventral (GiV) parts, participates in the induction of locomotion and muscle tone facilitation in decerebrate animals and receives moderate orexinergic innervation. In the present study, we have examined the role of orexin-A (OX-A) in muscle tone control using microinjections (50 microM, 0.3 microl) into the GiA and GiV sites in decerebrate rats. OX-A microinjections into GiA sites, previously identified by electrical stimulation as facilitating hindlimb muscle tone bilaterally, produced a bilateral increase of muscle tone in the same muscles. Bilateral lidocaine microinjections (4%, 0.3 microl) into the dorsolateral mesopontine reticular formation decreased muscle rigidity and blocked muscle tone facilitation produced by OX-A microinjections into the GiA sites. The activity of cells related to muscle rigidity, located in the pedunculopontine tegmental nucleus and adjacent reticular formation, was correlated positively with the extent of hindlimb muscle tone facilitation after medullary OX-A microinjections. OX-A microinjections into GiV sites were less effective in muscle tone facilitation, although these sites produced a muscle tone increase during electrical stimulation. In contrast, OX-A microinjections into the gigantocellular nucleus (Gi) sites and dorsal paragigantocellular nucleus (DPGi) sites, previously identified by electrical stimulation as inhibitory points, produced bilateral hindlimb muscle atonia. We propose that the medioventral medullary region is one of the brain stem target for OX-A modulation of muscle tone. Facilitation of muscle tone after OX-A microinjections into this region is linked to activation of intrinsic reticular cells, causing excitation of midbrain and pontine neurons participating in muscle tone facilitation through an ascending pathway. Moreover, our results suggest that OX-A may also regulate the activity of medullary neurons participating in muscle tone suppression. Loss of OX function may, therefore, disturb both muscle tone facilitatory and inhibitory processes at the medullary level.

DOI10.1111/j.1365-2869.2012.01023.x
Alternate JournalJ. Neurophysiol.