Total sleep deprivation increases extracellular serotonin in the rat hippocampus.

TitleTotal sleep deprivation increases extracellular serotonin in the rat hippocampus.
Publication TypeJournal Article
Year of Publication2003
AuthorsLopez-Rodriguez, F, Wilson CL, Maidment NT, Poland RE, Engel J
Date Published2003
KeywordsAnalysis of Variance, Animals, Chromatography, High Pressure Liquid, Electrochemistry, Electroencephalography, Electromyography, Extracellular Space, Hippocampus, Male, microdialysis, Rats, Rats, Sprague-Dawley, Serotonin, sleep deprivation, Sleep Stages, Stress, Physiological, Time Factors

Sleep deprivation exerts antidepressant effects after only one night of deprivation, demonstrating that a rapid antidepressant response is possible. In this report we tested the hypothesis that total sleep deprivation induces an increase in extracellular serotonin (5-HT) levels in the hippocampus, a structure that has been proposed repeatedly to play a role in the pathophysiology of depression. Sleep deprivation was performed using the disk-over-water method. Extracellular levels of 5-HT were determined in 3 h periods with microdialysis and measured by high performance liquid chromatography coupled with electrochemical detection. Sleep deprivation induced an increase in 5-HT levels during the sleep deprivation day. During an additional sleep recovery day, 5-HT remained elevated even though rats displayed normal amounts of sleep. Stimulus control rats, which had been allowed to sleep, did not experience a significant increased in 5-HT levels, though they were exposed to a stressful situation similar to slee-deprived rats. These results are consistent with a role of 5-HT in the antidepressant effects of sleep deprivation.

Alternate JournalNeuroscience