Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.

TitleCulture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.
Publication TypeJournal Article
Year of Publication2009
AuthorsEspinosa-Jeffrey, A, Wakeman DR, Kim SU, Snyder EY, de Vellis J
JournalCurrent protocols in stem cell biology
VolumeChapter 2
PaginationUnit 2D.4
Date Published2009 Sep
ISSN1938-8969
KeywordsAnimals, Animals, Newborn, Biological Assay, Cell Culture Techniques, Cell Differentiation, Cell Lineage, Cell Proliferation, Cell Separation, Cell Survival, Cryopreservation, Humans, Myelin Sheath, Neural Cell Adhesion Molecules, Neurons, Oligodendroglia, Rats, Stem Cell Transplantation, Stem Cells
Abstract

Here we document protocols for the production, isolation, and maintenance of the oligodendrocyte phenotype from rodent and human neural stem cells. Our unique method relies on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of oligodendrocytes as they advance from oligodendrocyte progenitors to mature, myelinating oligodendrocytes.

DOI10.1042/AN20110027
Alternate JournalCurr Protoc Stem Cell Biol