Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.
|Title||Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.|
|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Espinosa-Jeffrey, A, Wakeman DR, Kim SU, Snyder EY, de Vellis J|
|Journal||Current protocols in stem cell biology|
|Date Published||2009 Sep|
|Keywords||Animals, Animals, Newborn, Biological Assay, Cell Culture Techniques, Cell Differentiation, Cell Lineage, Cell Proliferation, Cell Separation, Cell Survival, Cryopreservation, Humans, Myelin Sheath, Neural Cell Adhesion Molecules, Neurons, Oligodendroglia, Rats, Stem Cell Transplantation, Stem Cells|
Here we document protocols for the production, isolation, and maintenance of the oligodendrocyte phenotype from rodent and human neural stem cells. Our unique method relies on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of oligodendrocytes as they advance from oligodendrocyte progenitors to mature, myelinating oligodendrocytes.
|Alternate Journal||Curr Protoc Stem Cell Biol|