Increases in amino-cupric-silver staining of the supraoptic nucleus after sleep deprivation.
|Title||Increases in amino-cupric-silver staining of the supraoptic nucleus after sleep deprivation.|
|Publication Type||Journal Article|
|Year of Publication||2002|
|Authors||Eiland, MM, Ramanathan L, Gulyani S, Gilliland M, Bergmann BM, Rechtschaffen A, Siegel JM|
|Date Published||2002 Jul 26|
|Keywords||Animals, cerebral cortex, Male, Rats, Rats, Sprague-Dawley, Reference Values, sleep deprivation, Staining and Labeling, Supraoptic Nucleus, Time Factors|
Sleep deprived rats undergo a predictable sequence of physiological changes, including changes in skin condition, increased energy expenditure, and altered thermoregulation. Amino-cupric-silver staining was used to identify sleep deprivation related changes in the brain. A significant increase in staining was observed in the supraoptic nucleus (SON) of the hypothalamus of rats with high sleep loss (>45 h) vs. their yoked controls. Follow-up experiments showed that staining was not significantly different in rats sleep deprived for less than 45 h, suggesting that injurious sleep deprivation-related processes occur above a threshold quantity of sleep loss. These anatomical changes suggest that the effects of sleep deprivation may be related to protein metabolism in certain brain regions.
|Alternate Journal||Brain Res.|