Increases in amino-cupric-silver staining of the supraoptic nucleus after sleep deprivation.

TitleIncreases in amino-cupric-silver staining of the supraoptic nucleus after sleep deprivation.
Publication TypeJournal Article
Year of Publication2002
AuthorsEiland, MM, Ramanathan L, Gulyani S, Gilliland M, Bergmann BM, Rechtschaffen A, Siegel JM
JournalBrain research
Volume945
Issue1
Pagination1-8
Date Published2002 Jul 26
ISSN0006-8993
KeywordsAnimals, cerebral cortex, Male, Rats, Rats, Sprague-Dawley, Reference Values, sleep deprivation, Staining and Labeling, Supraoptic Nucleus, Time Factors
Abstract

Sleep deprived rats undergo a predictable sequence of physiological changes, including changes in skin condition, increased energy expenditure, and altered thermoregulation. Amino-cupric-silver staining was used to identify sleep deprivation related changes in the brain. A significant increase in staining was observed in the supraoptic nucleus (SON) of the hypothalamus of rats with high sleep loss (>45 h) vs. their yoked controls. Follow-up experiments showed that staining was not significantly different in rats sleep deprived for less than 45 h, suggesting that injurious sleep deprivation-related processes occur above a threshold quantity of sleep loss. These anatomical changes suggest that the effects of sleep deprivation may be related to protein metabolism in certain brain regions.

DOI10.1111/j.1365-2869.2012.01023.x
Alternate JournalBrain Res.