Regulated expression of the Ras effector Rin1 in forebrain neurons.
|Title||Regulated expression of the Ras effector Rin1 in forebrain neurons.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Dzudzor, B, Huynh L, Thai M, Bliss JM, Nagaoka Y, Wang Y, Ch'ng TH, Jiang M, Martin KC, Colicelli J|
|Journal||Molecular and cellular neurosciences|
|Date Published||2010 Jan|
|Keywords||Animals, Cells, Cultured, Gene Expression Regulation, Genes, Reporter, Humans, Mice, Mice, Transgenic, Neurons, Promoter Regions, Genetic, Prosencephalon, rab GTP-Binding Proteins, Signal Transduction, Transcription Factors|
The Ras effector Rin1 is induced concomitant with synaptogenesis in forebrain neurons, where it inhibits fear conditioning and amygdala LTP. In epithelial cells, lower levels of Rin1 orchestrate receptor endocytosis. A 945 bp Rin1 promoter fragment was active in hippocampal neurons and directed accurate tissue-specific and temporal expression in transgenic mice. Regulated expression in neurons and epithelial cells was mediated in part by Snail transcriptional repressors: mutation of a conserved Snail site increased expression and endogenous Snai1 was detected at the Rin1 promoter. We also describe an element closely related to, but distinct from, the consensus site for REST, a master repressor of neuronal genes. Conversion to a consensus REST sequence reduced expression in both cell types. These results provide insight into regulated expression of a neuronal Ras effector, define a promoter useful in telencephalic neuron studies, and describe a novel REST site variant directing expression to mature neurons.
|Alternate Journal||Mol. Cell. Neurosci.|