Mass spectrometric measurements of the apolipoproteins of bovine (Bos taurus) HDL.

TitleMass spectrometric measurements of the apolipoproteins of bovine (Bos taurus) HDL.
Publication TypeJournal Article
Year of Publication2012
AuthorsDella Donna, L, Bassilian S, Souda P, Nebbia C, Whitelegge JP, Puppione DL
JournalComparative biochemistry and physiology. Part D, Genomics & proteomics
Volume7
Issue1
Pagination9-13
Date Published2012 Mar
ISSN1878-0407
KeywordsAmino Acid Sequence, Animals, Apolipoprotein A-I, Apolipoprotein C-III, Apolipoproteins, Apolipoproteins A, Cattle, Lipoproteins, HDL, Molecular Sequence Data, Spectrometry, Mass, Electrospray Ionization
Abstract

As is the case in most mammals, high density lipoproteins (HDL) also comprise the major group of lipid carriers that circulate in bovine (Bos taurus) blood. As a continuation of our proteogenomic studies of mammalian apolipoproteins, we have obtained molecular masses for several of the apolipoproteins associated with bovine HDL. The major apolipoprotein on the HDL surface is apoA-I, but other apolipoproteins were also detected. Using electrospray-ionization mass spectrometry (ESI-MS), we report on values for apolipoproteins, A-I, proA-I and A-II, as well as post-translationally modified apoA-I. Analyses of tryptic fragments did reveal the presence of apoA-IV and apoC-III. However, in contrast to our previous studies of other mammalian HDL, we did not detect apoC-I. Interestingly, examination of the current assembly for the bovine genome does not show any evidence for an apoC-I gene.

DOI10.1111/j.1600-0854.2011.01308.x
Alternate JournalComp. Biochem. Physiol. Part D Genomics Proteomics