Differential pulse voltammetry in vivo--evidence that uric acid contributes to the indole oxidation peak.
|Title||Differential pulse voltammetry in vivo--evidence that uric acid contributes to the indole oxidation peak.|
|Publication Type||Journal Article|
|Year of Publication||1983|
|Authors||Crespi, F, Sharp T, Maidment N, Marsden C|
|Date Published||1983 Dec 30|
|Keywords||Animals, Corpus Striatum, Hydroxyindoleacetic Acid, Male, Membrane Potentials, Rats, Rats, Inbred Strains, Synaptic Transmission, Uric Acid|
Previous studies using differential pulse voltammetry have shown that indoleamines contribute to the oxidation peak at +280-300 mV (peak 3) measured in the rat striatum in vivo using carbon fibre electrodes. In this study, using similar techniques, it is shown that 5-hydroxyindoleacetic acid and uric acid oxidize at a similar potential (+270-290 mV) in vitro. Additionally, by microinfusing uric acid or its metabolizing enzyme uricase, it is shown that uric acid oxidation contributes to about 30% of the height of peak 3 measured in the rat striatum in vivo. These results indicate that care needs to be taken in interpreting changes in the height of the in vivo peak 3 since it is not solely due to the oxidation of brain indoleamines.
|Alternate Journal||Neurosci. Lett.|